Knockout Procedures for Black Widow Spider

Dry Ice

This method is simple; the biggest complication may be acquiring dry ice. Care should be taken to prevent the dry ice from touching students or spiders.
1. Place portion cup with 5 ml of water in bottom of 600 ml beaker.
2. Carefully place the spider in the beaker, making sure that the spider doesn’t get into the 3. Place a pea-sized portion of dry ice into the portion cup and start the timer.
4. Place aluminum foil over the top, loosely molding it to make a cap.
5. Wait for spider to go limp & docile (approx. 1:45). Shake gently to check for response.
6. Remove spider by dumping out onto staging area. You may tape her down for feeding, silking, examination, etc.
• tape forelegs first to reduce chance of attack if she wakes up early

Baking Soda & Vinegar

This method is relatively simple, though it is more complicated than the burning candle process. Care must be taken to keep the spider out of the liquid.
1. Place 2.25 g of baking soda (NaHCO3) in bottom of pint jar.
2. Put spider in plastic boat, and use long tongs to place boat in bottom of jar. Make sure spider stays in boat.
3. Carefully add 32 ml of vinegar (5% Acetic Acid) into jar, avoiding the spider in the boat. 4. Start timer, then place lid on jar loosely.
Note: Reaction will occur, but the boat will float above, keeping the spider safely away from liquid.
4. Allow 2 mins. to transpire, then remove boat with spider and place on staging area. (Spider should be limp and unresponsive for about 2 mins.) You can tape her down for feeding, silking, examination, etc.
• tape forelegs first to reduce chance of attack if she wakes up early

Burning Candle

This method is relatively simple to perform, but gives mixed results.
Protocol:
1. Affix a candle (e.g. birthday) upright to the bottom of a 600 ml beaker using clay or wax.
2. Carefully place spider inside of beaker (she can crawl around inside).
3. Light match (or splint) and use to light candle inside of beaker, start timer.
4. Place aluminum foil over top, loosely molding it to make a cap.
5. Wait for spider to go limp & docile (approx. 1:30). Shake gently to check for response.
6. Remove spider by dumping out onto staging area. You may tape her down for feeding, silking, examination, etc.
• tape forelegs first to reduce chance of attack if she wakes up early

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